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KMID : 0385219960060010016
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Korean Journal of Gerontology
1996 Volume.6 No. 1 p.16 ~ p.22
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Formation of the α1-Adrenoceptor-G-protein complex by stimulation with α1-agonist in pig liver
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Yoo Soon-Moon
Han Ki-Jung
Choi Myung-Sub
Kwon Nyoun-Soo
Baek Kwang-Jin
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Abstract
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One of primary functions of the is to modulate calcium levels in cells. In transmembrane signaling transduction, the α1-Adrenoceptor activates a 69 kDa phospholipase C through novel Gh-proteins, which are suggested species specific. In the present study, a ternary complex of α1-agonis-adrenoceptor-G-protein was developed by stimulating membrane fractions of pig liver with α1-agonist, epinephrine, and then purified with heparin-agarose and wheat germ agglutinin-agarose affinity chromatography. Inclusion of α1-Adrenoceptors and 77 kDa Gh-like proteins in the ternary complex were confirmed by [3H]prazosin binding assay and photoaffinity labeling with [ -33P]GTP. The ternary complex was formed by stimulating with α1-agonist in pig liver. These results suggested that Gh-like proteins played roles as signal mediators of α1-adrenoceptor.
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KEYWORD
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G-protein, α1-Adrenoceptor, ternary complex, pig liver
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